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krt5 promoter  (Genecopoeia)


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    Genecopoeia krt5 promoter
    A and B , High (A, <t>KRT5+,</t> >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.
    Krt5 Promoter, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/krt5 promoter/product/Genecopoeia
    Average 94 stars, based on 1 article reviews
    krt5 promoter - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma"

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    Journal: bioRxiv

    doi: 10.64898/2026.01.28.702332

    A and B , High (A, KRT5+, >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.
    Figure Legend Snippet: A and B , High (A, KRT5+, >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.

    Techniques Used: Staining, Expressing

    A , Structure of Lenti-KRT5mCherry (L-KRT5mCherry). B , Co-localization of KRT5 immunostaining (KRT5, green) and mCherry (L-KRT5) expression (magenta). Orange, overlay. Counterstaining with DAPI (blue). Scale bar, 60 µm. C , Quantification of cells expressing both KRT5 and L-KRT5 (KRT5+ L-KRT5+), or only KRT5 (KRT5+) or L-KRT5+. D , Experimental design of isolation of cells expressing both L-KRT5mCherry and L-hUbC-GFP or L-hUbC-GFP alone. E, PCR detection of L-hUbC-GFP (GFP) and L-KRT5mCherry (mCherry) DNA in both KRT5+ (K5+) and KRT5- (K5-) cells. All error bars denote s.d.
    Figure Legend Snippet: A , Structure of Lenti-KRT5mCherry (L-KRT5mCherry). B , Co-localization of KRT5 immunostaining (KRT5, green) and mCherry (L-KRT5) expression (magenta). Orange, overlay. Counterstaining with DAPI (blue). Scale bar, 60 µm. C , Quantification of cells expressing both KRT5 and L-KRT5 (KRT5+ L-KRT5+), or only KRT5 (KRT5+) or L-KRT5+. D , Experimental design of isolation of cells expressing both L-KRT5mCherry and L-hUbC-GFP or L-hUbC-GFP alone. E, PCR detection of L-hUbC-GFP (GFP) and L-KRT5mCherry (mCherry) DNA in both KRT5+ (K5+) and KRT5- (K5-) cells. All error bars denote s.d.

    Techniques Used: Immunostaining, Expressing, Isolation

    A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.
    Figure Legend Snippet: A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

    Techniques Used: Derivative Assay, Expressing, Transplantation Assay, Microscopy, Isolation

    A. Effect of cisplatin and doxorubicin on KRT5+ and KRT5- cells. Manual count (cell viability, upper row) and MTT assay (absorbance at 590 nm, bottom row) of KRT5+ and KRT5- cells after 72 hours of treating with cisplatin and doxorubicin at different concentrations. B and C . Representative confocal images of primary HGSC organoids (B) and quantification of organoid frequency (B, left image) and size (C, right image) after treatment with different concentrations of cisplatin for 72 hours. C, Immunofluorescence for KRT5 (green). Counterstaining with DAPI (blue). Scale bar, 50 µm for all images. All error bars denote s.d.
    Figure Legend Snippet: A. Effect of cisplatin and doxorubicin on KRT5+ and KRT5- cells. Manual count (cell viability, upper row) and MTT assay (absorbance at 590 nm, bottom row) of KRT5+ and KRT5- cells after 72 hours of treating with cisplatin and doxorubicin at different concentrations. B and C . Representative confocal images of primary HGSC organoids (B) and quantification of organoid frequency (B, left image) and size (C, right image) after treatment with different concentrations of cisplatin for 72 hours. C, Immunofluorescence for KRT5 (green). Counterstaining with DAPI (blue). Scale bar, 50 µm for all images. All error bars denote s.d.

    Techniques Used: MTT Assay, Immunofluorescence

    A . Heat map depicting the top 200 differentially expressed genes in KRT5+ (A2, A1 and A4) and KRT5- (A7, A6, and A5) cells. Note the expression of SPP1 (osteopontin) in KRT5- cells (arrow). B and C . DAVID analysis for functional annotation of terms associated with KRT5+ (B) and KRT5- (C) cells.
    Figure Legend Snippet: A . Heat map depicting the top 200 differentially expressed genes in KRT5+ (A2, A1 and A4) and KRT5- (A7, A6, and A5) cells. Note the expression of SPP1 (osteopontin) in KRT5- cells (arrow). B and C . DAVID analysis for functional annotation of terms associated with KRT5+ (B) and KRT5- (C) cells.

    Techniques Used: Expressing, Functional Assay

    A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.
    Figure Legend Snippet: A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

    Techniques Used: Expressing, RNA Sequencing, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Transduction, shRNA



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    A and B , High (A, <t>KRT5+,</t> >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.
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    A and B , High (A, <t>KRT5+,</t> >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.
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    Image Search Results


    A and B , High (A, KRT5+, >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A and B , High (A, KRT5+, >40% stained neoplastic cells, n = 37) and low (B, KRT5-, n = 160) frequency of KRT5 expressing cancer cells. C , Kaplan-Meier survival analysis of HGSC patients stratified according to frequency of KRT5 expressing cells (P=0.0015). A and B, Elite ABC method. Hematoxylin counterstaining. Scale bar, 100 µm.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: Staining, Expressing

    A , Structure of Lenti-KRT5mCherry (L-KRT5mCherry). B , Co-localization of KRT5 immunostaining (KRT5, green) and mCherry (L-KRT5) expression (magenta). Orange, overlay. Counterstaining with DAPI (blue). Scale bar, 60 µm. C , Quantification of cells expressing both KRT5 and L-KRT5 (KRT5+ L-KRT5+), or only KRT5 (KRT5+) or L-KRT5+. D , Experimental design of isolation of cells expressing both L-KRT5mCherry and L-hUbC-GFP or L-hUbC-GFP alone. E, PCR detection of L-hUbC-GFP (GFP) and L-KRT5mCherry (mCherry) DNA in both KRT5+ (K5+) and KRT5- (K5-) cells. All error bars denote s.d.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A , Structure of Lenti-KRT5mCherry (L-KRT5mCherry). B , Co-localization of KRT5 immunostaining (KRT5, green) and mCherry (L-KRT5) expression (magenta). Orange, overlay. Counterstaining with DAPI (blue). Scale bar, 60 µm. C , Quantification of cells expressing both KRT5 and L-KRT5 (KRT5+ L-KRT5+), or only KRT5 (KRT5+) or L-KRT5+. D , Experimental design of isolation of cells expressing both L-KRT5mCherry and L-hUbC-GFP or L-hUbC-GFP alone. E, PCR detection of L-hUbC-GFP (GFP) and L-KRT5mCherry (mCherry) DNA in both KRT5+ (K5+) and KRT5- (K5-) cells. All error bars denote s.d.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: Immunostaining, Expressing, Isolation

    A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: Derivative Assay, Expressing, Transplantation Assay, Microscopy, Isolation

    A. Effect of cisplatin and doxorubicin on KRT5+ and KRT5- cells. Manual count (cell viability, upper row) and MTT assay (absorbance at 590 nm, bottom row) of KRT5+ and KRT5- cells after 72 hours of treating with cisplatin and doxorubicin at different concentrations. B and C . Representative confocal images of primary HGSC organoids (B) and quantification of organoid frequency (B, left image) and size (C, right image) after treatment with different concentrations of cisplatin for 72 hours. C, Immunofluorescence for KRT5 (green). Counterstaining with DAPI (blue). Scale bar, 50 µm for all images. All error bars denote s.d.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A. Effect of cisplatin and doxorubicin on KRT5+ and KRT5- cells. Manual count (cell viability, upper row) and MTT assay (absorbance at 590 nm, bottom row) of KRT5+ and KRT5- cells after 72 hours of treating with cisplatin and doxorubicin at different concentrations. B and C . Representative confocal images of primary HGSC organoids (B) and quantification of organoid frequency (B, left image) and size (C, right image) after treatment with different concentrations of cisplatin for 72 hours. C, Immunofluorescence for KRT5 (green). Counterstaining with DAPI (blue). Scale bar, 50 µm for all images. All error bars denote s.d.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: MTT Assay, Immunofluorescence

    A . Heat map depicting the top 200 differentially expressed genes in KRT5+ (A2, A1 and A4) and KRT5- (A7, A6, and A5) cells. Note the expression of SPP1 (osteopontin) in KRT5- cells (arrow). B and C . DAVID analysis for functional annotation of terms associated with KRT5+ (B) and KRT5- (C) cells.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A . Heat map depicting the top 200 differentially expressed genes in KRT5+ (A2, A1 and A4) and KRT5- (A7, A6, and A5) cells. Note the expression of SPP1 (osteopontin) in KRT5- cells (arrow). B and C . DAVID analysis for functional annotation of terms associated with KRT5+ (B) and KRT5- (C) cells.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: Expressing, Functional Assay

    A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

    Journal: bioRxiv

    Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

    doi: 10.64898/2026.01.28.702332

    Figure Lengend Snippet: A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

    Article Snippet: Briefly, for lentivirus packaging psPAX2 (Addgene, 12260), pMD2.G envelope plasmid (Addgene, 12259), and KRT5 promoter clone (GeneCopoeia, HRPM15909-LvPM02, mCherry) were employed.

    Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Transduction, shRNA